Detection of Acidovorax avenae subsp. citrulli using PCR and MALDI-TOF MS
نویسندگان
چکیده
منابع مشابه
Multilocus sequence typing reveals two evolutionary lineages of Acidovorax avenae subsp. citrulli.
Acidovorax avenae subsp. citrulli, causal agent of bacterial fruit blotch, has caused considerable damage to the watermelon and melon industry in China and the United States. Understanding the emergence and spread of this pathogen is important for controlling the disease. To build a fingerprinting database for reliable identification and tracking of strains of A. avenae subsp. citrulli, a multi...
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The aim of this study was to develop a SYBR Green-based real-time PCR assay for the rapid, specific, and sensitive detection of Acidovorax avenae subsp. citrulli, which causes bacterial fruit blotch (BFB), a serious disease of cucurbit plants. The molecular and serological methods currently available for the detection of this pathogen are insufficiently sensitive and specific. Thus, a novel SYB...
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The bacterium, Acidovorax avenae ssp. avenae causes several important plant diseases including bacterial stripe of rice, bacterial stalk rot of corn, bacterial leaf blight of oats, and red stripe of sugarcane and millet. Although the organism is seed-borne in rice, no reliable seed assay is available. A semiselective liquid medium based on d-sorbitol and l-pyroglutamic acid (SP medium), and two...
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ABSTRACT To assess the diversity of Acidovorax avenae subsp. citrulli, 121 strains from watermelon, cantaloupe, and pumpkin were compared using pulse field gel electrophoresis of SpeI-digested DNA and gas chromatographic analysis of fatty acid methyl esters. Twenty-nine unique DNA fragments resulted from DNA digestion, and 14 distinct haplotypes were observed. Based on cluster analysis, two sub...
متن کاملSimultaneous detection of Acidovorax avenae subsp. citrulli and Didymella bryoniae in cucurbit seedlots using magnetic capture hybridization and real-time polymerase chain reaction.
To improve the simultaneous detection of two pathogens in cucurbit seed, a combination of magnetic capture hybridization (MCH) and multiplex real-time polymerase chain reaction (PCR) was developed. Single-stranded DNA hybridization capture probes targeting DNA of Acidovorax avenae subsp. citrulli, causal agent of bacterial fruit blotch, and Didymella bryoniae, causal agent of gummy stem blight,...
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ژورنال
عنوان ژورنال: Journal of Electrophoresis
سال: 2012
ISSN: 1349-9394,1349-9408
DOI: 10.2198/jelectroph.56.13